Six-Pulse RIDME Sequence to Avoid Background Artifacts

Control experiments to avoid artifacts and misinterpretations in nanoecotoxicology testing

Artifacts in trimethylsilyl derivatization reactions and ways to avoid them.

Trimethylsilyl derivatives are routinely employed in gas chromatography to increase the volatility and stability of organic compounds containing active hydrogens. Normally only the desired derivative is formed when organic compounds are derivatized with common silylation reagents. However, some compounds form additional unexpected derivatives or by-products (artifacts). Artifact formation leads...

Simple Sequence Repeats Amplification: a Tool to Survey the Genetic Background of Olive Oils

A reliable DNA extraction method for use on extra virgin olive oil based on a commercial kit was defined, and the possibility of using this DNA for fingerprinting the original cultivar was demonstrated. The genetic traceability of single-cultivar virgin olive oil from two cultivars (Carolea and Frantoio) was achieved by identifying the varieties from which they were produced. This involved the ...

WE-G-217A-01: Fast Recovery Driven Equilibrium with Automated Switching to Avoid Artifacts.

PURPOSE To reduce image artifacts from the unnecessary use of driven- equilibrium. Driven-equilibrium methods known as fast recovery, RESTORE, or DRIVE enable reduction of repetition time (TR) and hence acquisition time in RARE (FSE or TSE) imaging by applying additional RF pulses following each echotrain to accelerate T1 recovery. Fast recovery (FR) techniques are most useful when TR is less t...

RIDME spectroscopy on high-spin Mn2+ centers.

Pulsed EPR dipolar spectroscopy is a powerful tool for determining the structure and conformational dynamics of biological macromolecules, as it allows precise measurements of distances in the range of 1.5-10 nm. Utilization of high-spin Mn2+ species as spin probes for distance measurements is of significant interest, because they are biologically compatible and endogenous in numerous biologica...